Bio-valorization of Tagetes floral waste extract in fabrication of self-healing Schiff-base nanocomposite hydrogels for colon cancer remedy.

The drastic boom in floriculture and social events in religious and recreational places has inevitably led to generation of tremendous floral waste across the globe. Marigold (Tagetes erecta) is one of the most common loose flowers offered for the same. Generally discarded, these Tagetes floral wastes could be valorized for biogenic syntheses. In this study, we have utilized the floral extract towards green synthesis of nano ZnO, the formation of which was affirmed from different analytical techniques. Bionanocomposite Schiff-base hydrogel composed of chitosan and dialdehyde pectin was fabricated by the facile strategy of in situ polymer cross-linking, and the ZnO nanoparticles were embedded in the hydrogel matrix. The hydrogel exhibited remarkable self-healing ability. The antioxidant and anti-inflammatory activities were enhanced owing to nano ZnO. Furthermore, it was hemocompatible and biodegradable. A controlled release drug profile for 5-fluorouracil from the hydrogel was accomplished in the colorectum. The exposure of the drug-loaded nanocomposite hydrogel demonstrated improved anticancer effects in HT-29 colon cancer cells. The findings of this study altogether put forth the successful biovalorization of Tagetes floral waste extract for colon cancer remedy.

In situ forming dialdehyde xanthan gum-gelatin Schiff-base hydrogels as potent controlled release fertilizers.

Controlled release fertilizer (CRF) hydrogels have blossomed into promising materials in agriculture owing to the sustained release of the fertilizer and also as soil conditioner. Apart from the traditional CRF hydrogels; Schiff-base hydrogels have garnered significant thrust that release nitrogen slowly in addition to reducing the environmental pollution. Herein, we have fabricated Schiff-base CRF hydrogels composed of dialdehyde xanthan gum (DAXG) and gelatin. The formation of the hydrogels was accomplished via the simplistic in situ crosslinking reaction between the aldehyde groups of DAXG and the amino groups of gelatin. The hydrogels acquired a compact network upon increasing the DAXG content in the matrix. The phytotoxic assay on different plants indicated the hydrogels to be nontoxic. The hydrogels demonstrated good water-retention behaviour in soil, along with reusability even after 5 cycles. A controlled release profile for urea was evident from the hydrogels wherein macromolecular relaxation played a crucial role in the release mechanism. Growth assays on Abelmoschus esculentus (Okra) plant presented an intuitive evaluation on the growth and water-holding capacity of the CRF hydrogel. The present work demonstrated a facile preparation of CRF hydrogels to enhance the utilization of urea and retain soil humidity as fertilizer carriers.

Harnessing the potential of dialdehyde alginate-xanthan gum hydrogels as niche bioscaffolds for tissue engineering.

Biomimetic hydrogels composed of natural polysaccharides have invariably blossomed as niche biomaterials in tissue engineering applications. The prospects of creating an extracellular matrix (ECM)-like milieu from such hydrogels has garnered considerable importance. In this study, we have fabricated bioscaffolds comprising dialdehyde alginate and xanthan gum and explored their potential use in tissue regeneration. The fabricated scaffolds displayed an interconnected porous network structure that is highly desirable for the aforesaid application. The scaffolds were endowed with good mechanical properties, thermostability, protein adsorption efficacy and degradability. Curcumin-loaded hydrogels exhibited appreciable antibacterial activity against E. coli. In vitro cytocompatibility studies revealed that the scaffolds promoted adhesion and proliferation of 3T3 fibroblast cells. The Western blot analysis of p53 gene indicated no growth arrest or apoptosis in 3T3 cells thus, signifying the non-toxic nature of the scaffolds. Furthermore, the ECM formation was confirmed via SDS-PAGE analysis. The overall results clearly validated these scaffolds as effectual biomaterials for tissue engineering applications.

Controlled delivery of ibuprofen from poly(vinyl alcohol)-poly(ethylene glycol) interpenetrating polymeric network hydrogels.

Hydrogels composed of poly(vinyl alcohol) (PVA) and poly(ethylene glycol) (PEG) were synthesized using glutaraldehyde as crosslinker and investigated for controlled delivery of the common anti-inflammatory drug, ibuprofen (IBF). To regulate the drug delivery, solid inclusion complexes (ICs) of IBF in ß-cyclodextrin (ß-CD) were prepared and added to the hydrogels. The ICs were prepared by the microwave irradiation method, which is more environmentally benign. The formation of IC was confirmed by various analytical techniques and the synthesized hydrogels were also characterized. Controlled release of drug was achieved from the hydrogels containing the ICs in comparison to the rapid release from hydrogels containing free IBF. The preliminary kinetic analysis emphasized the crucial role of ß-CD in the drug release process that influences the polymer relaxation, thereby leading to prolonged release. The cytotoxicity assay validated the hydrogels as non-toxic in nature and hence can be utilized for controlled delivery of IBF.

Correction: Non-thermal plasma assisted surface nano-textured carboxymethyl guar gum/chitosan hydrogels for biomedical applications.

[This corrects the article DOI: 10.1039/C8RA09161G.].

Non-thermal plasma assisted surface nano-textured carboxymethyl guar gum/chitosan hydrogels for biomedical applications.

Smart hydrogels comprising carboxymethyl guar gum and chitosan (CMGG/CS) have been fabricated using tetraethyl orthosilicate as the crosslinker. To render the hydrogels an improved biological efficacy, non-thermal plasma assisted surface modification have been performed using Ar, O2 and a mixture of Ar and O2 gases. Enhanced surface wettability was witnessed post-plasma treatment. AFM analyses revealed the topographical changes of the hydrogels at the nano-scale level without any adverse effect on their bulk physical structure. The hydrogels exhibited pH-responsive swelling with maximum swelling in neutral pH. The release of diclofenac sodium from the hydrogels confirmed their potential towards colon-targeted drug delivery. Excellent biofilm eradication features against E. coli was demonstrated by the hydrogels. Hemolytic assay on human RBCs affirmed their hemocompatibility. Moreover, the hydrogels were found to be remarkably biodegradable. Thus, non-thermal plasma assisted surface nano-textured CMGG/CS hydrogels can be efficaciously explored for their diverse applications in biomedicine.

Controlled delivery of ibuprofen from poly(vinyl alcohol)-poly (ethylene glycol) interpenetrating polymeric network hydrogels / 药物分析学报

Hydrogels composed of poly(vinyl alcohol) (PVA) and poly(ethylene glycol) (PEG) were synthesized using glutaraldehyde as crosslinker and investigated for controlled delivery of the common anti-inflammatory drug, ibuprofen (IBF). To regulate the drug delivery, solid inclusion complexes (ICs) of IBF in β–cyclodextrin (β–CD) were prepared and added to the hydrogels. The ICs were prepared by the microwave irradiation method, which is more environmentally benign. The formation of IC was confirmed by various analytical techniques and the synthesized hydrogels were also characterized. Controlled release of drug was achieved from the hydrogels containing the ICs in comparison to the rapid release from hydrogels containing free IBF. The preliminary kinetic analysis emphasized the crucial role of β–CD in the drug release process that in-fluences the polymer relaxation, thereby leading to prolonged release. The cytotoxicity assay validated the hydrogels as non-toxic in nature and hence can be utilized for controlled delivery of IBF.

Spectroscopic and computational insights into theophylline/ß-cyclodextrin complexation: inclusion accomplished by diverse methods.

Current scenario in asthmatic prevalence worldwide calls for a facile, cost-effective, and energy efficient methodology to formulate the potent bronchodilator, theophylline (THP), into an effective dosage forms. Since the uses of THP are severely impeded by its poor aqueous solubility and low bioavailability, solid inclusion complexes (ICs) of THP in ß-cyclodextrin (ß-CD) were prepared to overcome the limitations. The ICs were developed by conventional methods and also by microwave irradiation method, which is environmentally more benign and requires lesser reaction time. The complexation phenomenon was effectual by the co-precipitation, freeze-drying, and microwave methods as affirmed from various spectroscopic analyses. 1H NMR and molecular docking studies illustrated the total inclusion of THP into ß-CD cavity. Better efficacy of the microwaved product was witnessed in terms of drug content, dissolution, and anti-biofilm activities. Thus microwave irradiation can be utilised as a naive and economical methodology to design ß-CD-THP dosage formulations.

pH-Responsive guar gum hydrogels for controlled delivery of dexamethasone to the intestine.

pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and ß-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported. Hydrogels of different compositions were synthesized and evaluated for the controlled delivery of DX. The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD. Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels. As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged. The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine. Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.